PloS one
integrating single-cell transcriptomics and chromatin accessibility profiling to decipher the molecular landscape of Hepatocellular carcinoma
Von Pan K, Chen L und Zheng Y.
Inhalt auf Englisch
Abstract
Background
Hepatocellular carcinoma (HCC) is a prevalent and lethal liver malignancy, characterized by high recurrence rates and limited therapeutic options. Current diagnostic and treatment strategies are hindered by drug resistance and a lack of effective biomarkers for early detection.
Methods
This study aims to elucidate the role of lactylation and fibroblast cells within the tumor microenvironment (TME) of HCC, focusing on their potential as therapeutic targets. Utilizing a comprehensive multi-omics approach, we integrated single-cell RNA sequencing (scRNA-seq) and single-cell ATAC sequencing (scATAC-seq) data from public databases (GEO, TCGA) to explore transcriptional and epigenetic landscapes at single-cell resolution.