Advanced Pharmaceutical Bulletin
Magnetic graphene oxide improves the expression of some human cumulus cells biomarkers
Von Fahimeh Kabiri, Tahereh Foroutan, Roghiyeh Pashaei‐Asl und 3 weiteren.
Inhalt auf Englisch
Abstract
Purpose
Developing non-invasive, accurate methods for selecting high-implantation embryos is crucial for assisted reproductive technologies (ART). Cumulus cells (CCs) are reliable indicators of oocyte quality. This study investigated the effects of magnetic graphene oxide (MGO) on the viability, apoptosis, oxidative status, and proliferation-related proteins of human CCs in vitro.
Methods
Human CCs were treated with MGO (1.5–400 µg/mL) for 24, 48, and 72 hours. Viability was assessed via MTT and Annexin V-FITC/PI flow cytometry. Expression of apoptosis-related (Bcl-2, Bax, cleaved/pro-caspase-3), antioxidant (Nrf2, SOD2), and cell-cycle (cyclin D1) proteins was analyzed using Western blotting. Additionally, Catalase (CAT) activity, Reduced glutathione (GSH), and total antioxidant capacity (TAC) were determined using colorimetric assays.